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CABRI:ICLC |
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Accession_number |
ICLC HTL04001
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Cell_line_name |
293T
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Brief_description |
Species: human; Tissue: kidney, embryonic; Transformed by: SV40; large T antigen; Original line: 293
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Description |
Species: human; Tissue: kidney, embryonic; Transformed by: SV40; large T antigen; Original line: 293
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Depositor |
Dr R. Notaro, Human Genetics Dept IST, Genoa, Italy
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Bibliographic_reference |
-
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Morphology |
epithelial-like, grown as monolayer
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Culture_conditions |
continuous culture; DMEM + 20% FBS + 2mM L-Glutamine; Split subconfluent cultures (70% confluence)1:4-1:8 using trypsin/EDTA; 37°C, 5% CO2
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Viruses |
Search for viruses was not performed
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Properties |
SV40 large T antigen expression. Used for transfection assays and as packaging cells. The cell line can be transiently transfected and give extremely high levels of expression of AP fusion proteins. G418 resistant, neomicine resistant. The cell line is known also as 293tsA1609neo.
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
Handle under Biosafety Level 2 containment
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Freezing_medium |
90% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Validation_assays |
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
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Further_bibliography |
Mol Cell Biol 1987;7:379-387 [PMID: 3031469
]
Proc Natl Acad Sci USA 1993;90:8392-8396 [PMID: 7690960
]
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Comments |
The cells detach easily with PBS, but trypsin is necessary to disaggregate the cells
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